FIGURE 5.

The role of β-arrestin 2 in antibody recognition. A, PFC membranes from β-arrestin 2 knock-out mice (β-arr k/o) or their wild-type (WT) littermate controls were treated with 1 μm deltorphin II for 30 min at 37 °C and the extent of antibody recognition by δOR (5A2) mAb monitored by ELISA as described under “Experimental Procedures.” B, β-arrestin 2 knock-out mice (β-arr k/o) or their WT littermate controls were injected intraperitoneally with either 10 mg/kg morphine, 1 mg/kg naloxone, 10 mg/kg morphine + 1 mg/kg naloxone, or saline and sacrificed 30 min later. Membranes prepared from the PFC were probed by ELISA with the μOR (4D6) mAb as described under “Experimental Procedures.” Receptor levels were determined by incubating membranes (10 μg) with 1 nm [³H]DAMGO as described under “Experimental Procedures.” Nonspecific binding was determined in the presence of 1 μm DAMGO. Results are mean ± S.E. of three experiments from 3-4 animals. ^**, p < 0.01 versus control, Dunnett's test.