Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
The zinc finger of LigIII contributes DNA binding affinity and specificity. Binding of LigIII (A), ΔZnF (B), and ZnF (C) to DNA was measured by fluorescence anisotropy in buffer containing 250 mm NaCl. The DNA substrate was an intact duplex (○) or contained a single-stranded nick (•). Nicked DNA lacking the 3′-hydroxyl (▾) or the 5′-phosphate (▵) was also assayed.
