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Effect of nick structure on DNA binding: DNA binding affinities (KDNA, μm) measured in 250 mm NaCl
| Ligatable nick | ddC | No 5′ PO4 | |
|---|---|---|---|
| LigIII | 0.30 ± 0.08 | 0.11 ± 0.02 | 2.8 ± 0.27 |
| ZnF-DBD | 2.5 ± 1.8 | 9.8 ± 2.3 | 5.1 ± 3.1 |
| ΔZnF | 0.63 ± 0.24 | 0.87 ± 0.15 | >30 |
| CC | 0.46 ± 0.15 | 2.4 ± 0.7 | >30 |
