FIGURE 11.
ROS scavenger rescues HIF-1α-deficient MEFs from hypoxia-induced cell death. MEFs were exposed to 20 or 1% O2 for 48 h in the presence of 25 μm MnTMPyP, a superoxide dismutase mimetic, or vehicle control (CTR). A, ROS levels were quantified by dichlorofluorescein (DCF) fluorescence. B, percent cell death (mean ± S.E.) was quantified by trypan blue staining. *, p < 0.05 by Student's t test compared with WT-CTR at 20% O2;#, p < 0.05 compared with WT-CTR at 1% O2; **, p < 0.05 for indicated comparison. C, molecular pathway regulating mitochondrial autophagy, cell respiration, ROS levels, and cell survival in MEFs subjected to prolonged hypoxia.