HIF-1-dependent induction of BNIP3 expression in hypoxic MEFs.
A, BNIP3 mRNA was measured by quantitative real-time RT-PCR in WT and
KO MEFs exposed to 20 or 1% O2 for 24 h. Mean values (±S.E.)
are shown. *, p < 0.05 by Student's t test
compared with WT MEFs at 20% O2;#, p < 0.05 compared
with WT MEFs at 1% O2. B, BNIP3 and β-actin protein
expression was measured by immunoblot assay using lysates from WT and KO MEFs
exposed to 20 or 1% O2 for 48 h. C and D, BNIP3
mRNA (C) and protein (D) expression were analyzed in WT and
HET mouse lung tissues. *, mean (±S.E., n = 3) that
is significantly different from WT. Anti-tubulin immunoblot assay was
performed to confirm equal protein loading. E and F, BNIP3
mRNA (E) and protein (F) levels were analyzed in
Cre- and Cre+ mouse lung tissues. *, mean
(±S.E., n = 3) that is significantly different from
Cre-. Tubulin served as loading control. NS indicates a
nonspecific band.