FIGURE 8.

BNIP3 competes with Beclin-1 for binding to Bcl2. A, MEFs were exposed to 20 or 1% O₂ for 48 h, whole cell lysates (WCL) were prepared, and aliquots were subjected to direct immunoblot assays (IB, left panel) or after immunoprecipitation (IP) with anti-Bcl2 antibody (right panel). B and C, MEFs stably transfected with EV or vector encoding Bcl2 were exposed to 20 or 1% O₂ for 48 h and analyzed for mitochondrial:nuclear DNA ratio (B) or punctate fluorescence of GFP-LC3 (C). Mean data (±S.E.) are shown. ^*, p < 0.05 compared with WT-EV at 20% O₂;#, p < 0.05 compared with WT-EV at 1% O₂.