FIGURE 5.

Depletion of Fra1 does not affect mitochondrial aconitase. A, fra1-1 cells containing a FET3-lacZ reporter construct were transformed with a plasmid containing a β-estradiol-inducible FRA1. The cells were grown in CM in the presence of β-estradiol, and then either cells were maintained with β-estradiol, or β-estradiol was removed for the specified times. Samples were taken, and Fra1-His₆ protein was determined by Western blot analysis. Aliquots were also taken to assay β-galactosidase (B) and aconitase (C) activity.