FIGURE 1.

Sensitivity of AngII-stimulated pMnk1 activation to kinase inhibitors. A, HEK-293 cells stably expressing HA-AT1_AR and Mnk1 were serum-starved for 16 h, pretreated with vehicle Me₂SO (DMSO) or the indicated kinase inhibitor for 30 min, and then stimulated with 100 nm AngII for the indicated times. Western blots for phospho-Mnk1 and tMnk1 were performed. The percentage of pMnk1/tMnk1 was calculated for each data point and plotted as a percentage of maximal activity (Me₂SO-treated cells at 30 min) ± S.E. These data represent the average ± S.E. of seven independent experiments. B, representative pMnk1 Western blots. C, means of individual data points (as a percent of maximal stimulation) up to 30 min from experiments performed in A. D, calculations based on addition or subtraction of means from A as shown in the legend. Solid lines in C represent actual data; dashed lines in D represent mathematically calculated values.