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. 2008 Apr 18;283(16):10967–10977. doi: 10.1074/jbc.M709741200

FIGURE 3.

FIGURE 3.

Nucleotide sequences of the upstream regions of SDHa, SDHb, SDHc, and SDHd in H9c2 cells. A, consensus sequences for transcription factor binding located with DNASIS (Hitachi Software; Alameda, CA) and MatInspector (Genomatix Software; München, Germany). Putative NRF-1 and NRF-2 canonical binding sites of 92-100% homology are underlined (EntrezGene data base ID numbers SDHa 157074, SDHb 298596, SDHc 289217, SDHd 363061). The arrows indicate major transcription start sites. Uppercase letters indicate promoter and intron sequences, and lowercase letters are exon sequences. a, SDHa 5′-region within the 180-nt upstream sequence and the NRF-1 sites underlined; b, SDHb 5′-region within the 180-nt upstream sequence and NRF-2 sites underlined; the underlined sites at -90 to -77 is a partial NRF-1 consensus sequence; c, SDHc 5′-region within the 180-nt upstream sequence, the NRF-2 site is underlined; no NRF-1 site is found; d, SDHd 5′-region within the 180-nt upstream sequence, the NRF-2 sites and single NRF-1 site are underlined. B, ChIP assay for NRF-1 binding to the SDHa, SDHb, SDHc, and SDHd promoters. H9c2 cells were transfected with scRNA or siRNA directed at NRF-1 or exposed to 100 μm DCM/CO or medium alone and subjected to ChIP assays using anti-NRF-1. Input lanes show chromatin PCR product before immunoprecipitation. Precipitated DNA was analyzed by PCR with specific primer sets. Data are from one of three experiments.