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. 2008 Apr 18;283(16):10967–10977. doi: 10.1074/jbc.M709741200

FIGURE 5.

FIGURE 5.

SDHa silencing increases HIF-1 target gene expression in H9c2 cells. A, Western blot analysis of glucose transporter proteins Glut1, Glut2, and Glut4 in control lysate (lanes 1 and 2) or lysate from cells transfected with siRNA to SDHa. Lanes 3, 4, lanes 5, 6, and lanes 7, 8 show timed effects of SDHa siRNA on transporter levels. Tubulin is the loading control. The histogram shows Glut 1, 2, and 4 protein levels by densitometry (*, p < 0.05 compared with control; † indicates p < 0.05 compared with control and Glut1). B, Western blot analysis for HO-1 in H9c2 cell lysates described in A shows protein induction after SDHa silencing. (Tubulin is for comparison.) The histogram shows relative HO-1 induction by densitometry (n = 3; *, p < 0.05 compared with control). C, siNRF-1 causes the expected increases in Glut4 and HO-1 protein by Western blot analysis by 24 h. In duplicate experiments, densitometry indicated that both proteins increased after siNRF-1 by an amount comparable to siSDHa (∼2-fold and 3-fold respectively; p < 0.05 for both).