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. 2008 Apr 27;36(Web Server issue):W35–W41. doi: 10.1093/nar/gkn211

Table 1.

Web servers related to PVS

Web server Input: formats Output and tasks Ref
• PVS http://imed.med.ucm.es/PVS/ • MSA: CLUSTAL, FASTA, GCG/MSF
• PDB: Uploaded or retrieved
• MSA and PDB
1. Compute sequence variability
2. Plot sequence variability
3. Map and display variability in 3D structures
4. Mask sequence variability
5. T-cell epitope prediction
6. Return conserved fragments
7. Locate conserved fragments into 3D structures/B-cell epitope prediction
• SVS* http://bio.dfci.harvard.edu/Tools/svs.html • MSA: CLUSTAL 1. Compute sequence variability as given by Shannon Entropy
2. Plot sequence variability
3. Return conserved fragments
• SiteVarProt http://159.149.109.16/Tools/SiteVarProt.php • MSA: FASTA 1. Compute relative sequence variability
2. Plot sequence variability
(35)
• Consensus http://coot.embl.de/Alignment//consensus.html • MSA: CLUSTAL and GCG/MSF 1. Consensus sequence at various thresholds with amino acid groupings
• Conseq http://conseq.bioinfo.tau.ac.il/ • SEQUENCE: FASTA
• MSA: NBRF/PIR, EMB, FASTA, GDE,  CLUSTAL, GCG/MSF and RSF
1. Compute conservation scores
2. Compute solvent accessibility
3. Return color-coded sequence with calculations
(9)
• Consurf http://consurf.tau.ac.il/ • PDB: Uploaded or retrieved
• MSA and PDB
1. Compute conservation scores
2. Map and display conservation scores in 3D structures
(11)
• TreeDet http://www.pdg.cnb.uam.es/Servers/treedet/ • MSA: CLUSTAL, FASTA, MSF and PIR • Predicts and display functionally relevant residues (10)
• Conservancy http://tools.immuneepitope.org/tools/conservancy • SEQUENCES: FASTA • Computes per site sequence identity of epitopes in protein sources (36)

PVS is an enhanced version of SVS, a server previously developed by Dr Reche. SVS has >85 000 hits since it started running in 2002.