Table 1.

Completeness and diversity statistics for two hypothetical site-saturation mutagenesis libraries, in which two codons have been randomized according to different schemes (NNK + NDT or NNB + NAY)

Library	No. of clones sampled	GLUE		GLUE-IT
		No. of distinct DNA variants	DNA completenessa	No. of distinct amino acid variantsb	Amino acid completenessc
NNK + NDT	100	88	0.23	77	0.32
	500	280	0.73	196	0.82
	1000	356	0.93	229	0.95
	1500	376	0.98	237	0.99
NNB + NAY	100	88	0.23	53	0.66
	500	280	0.73	78	0.98
	1000	356	0.93	80	1.00
	1500	376	0.98	80	1.00

^aThe fraction of all possible DNA sequence variants (384 for each library) that are represented in the sample.

^bNot including variants with stop codons.

^cThe fraction of all possible amino acid variants (240 for Library 1; 80 for Library 2) that are sampled.