Figure 2.

A comparison between the prediction and experimental result. In blue is the experimental result (inhibition efficacy) for an mRNA target, Human Cyclophilin (Genbank ID: M60857), at 37°C (3). The inhibition efficacy is defined as 100% minus the percent level of mRNA expression after siRNA application as compared to a control. In red is the probability (ranging from 0–1) of being efficient (having inhibition efficacy larger than 70%), as predicted by OligoWalk. Position is the starting position of each siRNA on the target sequence.