Skip to main content
. Author manuscript; available in PMC: 2008 Jul 9.
Published in final edited form as: DNA Repair (Amst). 2007 Jun 29;6(11):1572–1583. doi: 10.1016/j.dnarep.2007.05.004

Figure 2.

Figure 2

Figure 2

Defining separate Exo1p PRR and MMR domains. (A) Schematic and summary of phenotypes for Exo1p deletion mutants tested by MMS sensitivity (PRR) and for MMR mutator as shown in Figure 2B and Table 2, respectively. Exo1p with functional domains and motifs as indicated by respective hatched boxes. Site-specific mutations experimentally examined are highlighted by asterisks as detailed in the text. This analysis defines Exo1p deletion mutant #4 (1-438 aa) as a separation of function mutant. (B) Complementation of MMS tolerance in an exo1Δ rev3Δ strain with the listed Exo1p deletion mutant constructs suggests that residues 1-438 are necessary for functional PRR. MMS sensitivity was performed as described previously in Figure 1.