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. Author manuscript; available in PMC: 2008 Jul 9.

Published in final edited form as: DNA Repair (Amst). 2007 Jun 29;6(11):1572–1583. doi: 10.1016/j.dnarep.2007.05.004

The exo1-FF447AA mutant is functional for PRR. Overnight saturated cultures were serially diluted (1:5), spotted on YPD plates and YPD plates with increasing concentrations of MMS using a 48-prong replicator and then incubated at 30 °C for 2 days. The exo1-FF447AA mutation shows no defect in the PRR pathway as this mutant is as resistant to MMS as wildtype and does not show any genetic interaction with the redundant (A) REV3- or (B) RAD9-dependent branches of PRR.

The exo1-FF447AA mutant is functional for PRR. Overnight saturated cultures were serially diluted (1:5), spotted on YPD plates and YPD plates with increasing concentrations of MMS using a 48-prong replicator and then incubated at 30 °C for 2 days. The exo1-FF447AA mutation shows no defect in the PRR pathway as this mutant is as resistant to MMS as wildtype and does not show any genetic interaction with the redundant (A) REV3- or (B) RAD9-dependent branches of PRR.