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. 2008 Jul 23;3(7):e2769. doi: 10.1371/journal.pone.0002769

Figure 4. Less apoptosis of epithelial cells surrouding PP areas in Nod2−/− mice.

Figure 4

Nod2−/− and Nod+/+mice were orogastrically inoculated with 1×107 CFU of YPIII(pIB102) bacteria and sacrificed 5 days after inoculation. Their intestines were removed and analyzed.(a) Cell death measured by the proportion of Trypan blue or Ethidium homodimer-1 positive cells was lower in the PPs of Nod2−/− mice (Student t-test). (b) Apoptosis measured by the number of caspase-3 stained epithelial cells in the 100 intestinal villi and crypts surrounding PPs was also lower in Nod2−/− mice (Student t-test). Photos show representative caspase-3 staining of intestinal villi and crypts. Error bars indicate mean+/−SEM. *P<0.05, **P<0.01, ***P<0.001.