Figure 5. Fluorescence-based microscopic evaluation of the effect of squalamine on bacterial integrity and survival.

E. Coli cultures (10⁹/mL) were either left untreated (A) or treated with increasing squalamine concentrations: 1.25 µg/mL (B), 2.5 µg/mL (C), 5 µg/mL (D), 10 µg/mL (E), 100 µg/ml (F). Suspensions of cultured E. Coli were then stained with the Live/Dead BacLight bacterial Viability kit, as described in material and methods. Scale bar (10 µm).