Table 4.
Enzyme | 1x γ-ECS + PCS | 2.5x γ-ECS + PCS | ||||||
---|---|---|---|---|---|---|---|---|
CviJGSH | CviJPC | CviGSH | CviPC | CviJGSH | CviJPC | CviGSH | CviPC | |
γ-ECS | 0.58 | 0.60 | 0.68 | 0.76 | 0.45 | 0.61 | 0.70 | 0.60 |
GS | <0.01 | <0.01 | 0.01 | 0.01 | 0.19 | <0.01 | <0.01 | 0.97 |
GS-transferase | 0.01 | −0.06 | −0.07 | −0.07 | <0.01 | <0.01 | < −0.01 | −0.05 |
PCS | 0.40 | 0.44 | −0.63 | −0.56 | 0.33 | 0.44 | −0.62 | 0.57 |
vacuole PC-Cd transporter | <0.01 | <0.01 | <0.01 | −1.2 | <0.01 | <0.01 | <0.01 | −2.1 |
CviJGSH, control coefficient of enzyme i in GSH synthesis; CviJPC, control coefficient of enzyme Ei on PCs synthesis; CviGSH, control coefficient of enzyme i on GSH concentration; CviPC, control coefficient of enzyme i on PCs concentration. An enzyme with a negative flux control indicates that it is localized in a branch, turning aside the principal flux; an enzyme with a negative concentration control indicates that an increase in its activity decreases metabolite concentration.