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. 2008 Jul 14;182(1):117–127. doi: 10.1083/jcb.200804023

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© 2008 Paschen et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 5. — CPAF expression leads to changes in cellular morphology. (A) Changes in cell morphology during CPAF expression. CPAF K6 cells were incubated either with 6 ng/ml AHT, CM, or both for 16 h. Cells were analyzed by light microscopy (left). Arrows indicate smaller vesicular fragments. Black bar, 10 μm. The right panel shows an enlarged section of either a control sample or a sample treated with AHT and CM. White bar, 3 μm. (B) Inhibition of CPAF oligomerization by novobiocin. CPAF expression was induced in CPAF K6 cells by adding AHT, or cells were left untreated. 30 min before addition of CM, the cells were incubated with indicated amounts of NB. Cells were analyzed by light microscopy. Bar, 10 μm.