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. 2008 Jul 14;182(1):129–140. doi: 10.1083/jcb.200711112

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© 2008 Geng et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 2. — More Cvt vesicles were formed in Atg11-overexpressing cells. Cells (pep4Δ vps4Δ CuApe1; JGY089) expressing either pAtg11, pCuAtg8, and pCuAtg19 or empty vectors were cultured in SMD medium to mid-log phase. Cells were harvested and prepared for EM as described in Materials and methods. Representative images of TEM and IEM are shown in A and B, respectively. Areas with typical Cvt vesicles are shown with higher magnification at the right. In A, the Cvt vesicles were drawn in black in the far right. Bars: (A) 500 nm; (B) 200 nm. (C and D) Quantification of Cvt body accumulation. The number was determined from cells with clearly defined Cvt bodies inside the vacuole.