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. 2008 Jul 14;182(1):129–140. doi: 10.1083/jcb.200711112

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© 2008 Geng et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 3. — Linear relationship between GFP-tagged protein amount and fluorescence intensity. The standard curve showed the relationship between protein amount and fluorescence intensity. Fluorescence intensity and GFP-tagged protein amount were quantified in the following strains: (a) P_ATG27GFP-Atg8; (b) Mlc2-GFP; (c) P_ATG27GFP-Atg8 pep4Δ; (d) P_VPS30-ATG18GFP-Atg8; (e) P_VPS30-ATG18GFP-Atg8 pep4Δ; (f) P_ATG3GFP-Atg8; (g) P_ATG3GFP-Atg8 pep4Δ; (h) P_ATG8GFP-Atg8; (i) Cdc12-GFP; and (j) P_ATG8GFP-Atg8 pep4Δ. Error bars indicate the SD of three independent experiments. For fluorescence intensity, >200 cells of each strain were analyzed by microscopy as described in Materials and methods. Linear regression was performed to generate the formula y = 11.029x.