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. 2008 Jul 14;182(1):129–140. doi: 10.1083/jcb.200711112

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© 2008 Geng et al.

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Figure 6. — Amount of Atg proteins at the PAS during either the Cvt pathway or autophagy. (A) Boxplot of the amount of Atg proteins at the PAS in growing conditions. The local protein amount was calculated as described in Materials and methods and graphed into a boxplot (n = 100). (B) Difference in the PAS intensity between the Cvt pathway and autophagy. GFP-tagged strains were cultured in nutrient-rich conditions (YPD medium; n = 100) and then shifted to starvation conditions (SD-N medium; n = 60) for 2 h. Microscopy was done before and after starvation. Error bars indicate the SEM of three independent experiments.