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. 2008 Jul 14;182(1):129–140. doi: 10.1083/jcb.200711112

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© 2008 Geng et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 7. — Kinetics of Atg proteins at the PAS during autophagy. GFP-tagged strains were starved for 2 h and analyzed by microscopy. (A) For Atg9-GFP, the PAS intensity remained as a relative constant for 30 min, but only nine time points are shown. Fluorescence intensity at time 0 was normalized to 1 (n = 9). Essentially the same results were seen for Atg1-, Atg11-, Atg16-, Atg17-, and Atg19-GFP. (B) For GFP-Atg8, the PAS intensity changed over time. The time point at which the punctate structure could first be seen was set as time 0 and fluorescence intensity at this time point was set to 1 (n = 11). In A and B, all data points came from three independent experiments and error bars indicate the SEM. (C) Representative images of GFP-Atg8 showing the change of PAS (arrow) intensity. Bar, 2 μm.