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. 2008 Jul 14;182(1):77–88. doi: 10.1083/jcb.200804062

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© 2008 Woolner et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 4. — Spindle movement is attenuated in Myo10 morphants. (a) Stills taken from a short section of a confocal movie of GFP–α-tubulin in a control embryo (Video 1, available at http://www.jcb.org/cgi/content/full/jcb.200804062/DC1). Time stamps indicate time in minutes and seconds. The metaphase spindle undergoes a sudden movement between t = 1:00 and t = 2:00; this “jerky” rotational movement is characteristic of metaphase spindles in control embryos at embryonic stages 9 and 10. (b) Stills taken from confocal movie of GFP–α-tubulin in a Myo10 morphant (Video 2) at the same embryonic stage as the control embryo in panel a. The morphant spindle, even while still bipolar, displays a much more gradual rotational movement than the control spindle. (c) A graph displaying the rotational movement of a control spindle compared with a Myo10 morphant spindle. The time at which spindles enter metaphase and anaphase or undergo pole fragmentation are indicated by arrows and accompanying labels.