Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2008 Jul 14;182(1):77–88. doi: 10.1083/jcb.200804062

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2008 Woolner et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 5. — Rescue experiments reveal that the head and tail of Myo10 mediate different aspects of Myo10 function in mitosis. (a) Schematic diagram of the constructs used in Myo10 morphant rescue experiments. (b) Low-magnification images of α-tubulin staining in rescue experiment embryos. For these experiments, embryos were microinjected with water or Myo10 MO along with RNA encoding each of the Myo10 constructs shown in panel a. (c) Quantification of bipolar and multipolar spindles in rescue experiment embryos. n = 18, 47, 22, 16, and 12 embryos for water, MO, MO + GFP-Myo10, MO + GFP-Myo10-HMM, and MO + GFP-Myo10-IQT, respectively. Error bars represent the standard error of the mean. (d) Box and whisker plots of spindle length measurements in rescue experiment samples. Spindle length was calculated as a percentage of total cell length to allow for variation in cell size. n = 109, 141, 136, 115, and 87 spindles for water, MO, MO + GFP-Myo10, MO + GFP-Myo10-HMM, and MO + GFP-Myo10-IQT, respectively. For significance testing, unpaired Student's t tests were performed: *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.