Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2008 Jul 14;182(1):61–76. doi: 10.1083/jcb.200803111

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2008 Pawlicki and Steitz

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 8. — Localization of endogenous pri-miRNAs. (A) Pri-miRNAs expressed at low levels were detected using amplification and localize to one or two foci per cell. (B) Pri-miR-34a is not detected in untreated HeLa cells (a–d) but shows diffuse nucleoplasmic localization in HeLa cells after UV irradiation (e–h). Costaining for SC35 is shown in panels b and f. (C) BIC/pri-miR-155 localizes throughout the nucleoplasm in Raji (a–d) and B95-8 cells treated with PMA and ionomycin (P/I) for 24 h, as well as in nuclear foci in B95-8 cells (e–h), many of which overlap with SC35 (g, arrows). (D) Pri-miR-BHRF1-2∼3 shows diffuse nucleoplasmic and cytoplasmic distribution in Raji cells (a–d) but is concentrated in nuclear foci in B95-8 cells (e–h), some of which overlap with SC35 (g, arrows). (E) EBV BHRF1 DNA localizes diffusely in the nucleus and cytoplasm (due to viral release). Bars, 10 μm.