TABLE 3.
Transcripts identified as differentially expressed with the use of the false discovery rate correction (P = 6 × 10-4) in muscle of older adults who consumed a diet with inadequate protein for 1 wk
| GenBank ID1 | Name | Ratio2 | |
|---|---|---|---|
| Immune, stress, and inflammatory response | NM_000745 | Cholinergic receptor, nicotinic, alpha polypeptide 5 | 1.90 |
| NM_000079 | Cholinergic receptor, nicotinic, alpha polypeptide 1 | 1.56 | |
| BE903880 | CD44 antigen | 1.89 | |
| Z22969 | CD163 antigen | 2.37 | |
| NM_017801 | Chemokine-like factor super family 6 | 1.41 | |
| Transport | U51478 | ATPase, Na+/K+ transporting, beta 3 polypeptide" | 1.45 |
| Cytoskeleton | NM_005720 | Actin related protein 2/3 complex, subunit 1B, 41 kDa | 1.77 |
| BC002654 | Tubulin, beta 6 | 1.68 | |
| Extracellular connective tissue | K01228 | Collagen, type I, alpha 1 (2×)3 | 5.61 |
| NM_000089 | Collagen, type I, alpha 2 (2×)3 | 4.02 | |
| AA292373 | Collagen, type VI, alpha 1 | 1.54 | |
| AU144167 | Collagen, type III, alpha 1 (3×)3 | 3.16 | |
| AL575735 | Collagen, type V, alpha 2 (2×)3 | 2.20 | |
| Contraction, movement, and development | NM_002475 | Myosin alkali light chain 1 slow a | 0.80 |
| NM_013292 | Myosin light chain 2 | 0.85 | |
| NM_020349 | Ankyrin repeat domain 2 | 0.89 | |
| NM_003118 | Secreted protein, acidic, cysteine-rich | 1.92 | |
| NM_002966 | S100 calcium binding protein A10 | 1.49 | |
| NM_004039 | Annexin A2 | 1.56 | |
| NM_002345 | Lumican | 1.58 | |
| AI826799 | EGF-containing fibulin-like extracellular matrix protein 1 | 1.79 | |
| NM_005979 | S100 calcium binding protein A13 | 1.71 | |
| NM_000138 | Fibrillin 1 (Marfan syndrome) | 1.66 | |
| BC000055 | Follistatin-like 1 | 1.81 | |
| U37283 | Microfibrillar associated protein 5 | 1.50 | |
| BC005858 | Fibronectin 1 (4×)3 | 2.43 | |
| NM_014624 | S100 calcium binding protein A6 | 1.49 | |
| NM_025008 | Thrombospondin repeat containing 1 | 1.54 | |
| BF218922 | Chondroitin sulfate proteoglycan 2 (2×)3 | 2.20 | |
| Mineral metabolism | AF333388 | Metallothionein 1H | 0.67 |
| NM_005952 | Metallothionein 1X (2×)3 | 0.65 | |
| BG538564 | Ferritin, light polypeptide | 1.49 | |
| Kinases and signaling | NM_005627 | Serum/glucocorticoid regulated kinase | 0.71 |
| NM_004165 | Ras-related associated with diabetes (2×)3 | 3.09 | |
| AA731713 | Dendrin | 1.45 | |
| AF245505 | Adlican | 1.89 | |
| Metabolism | M24317 | Alcohol dehydrogenase 1C (class I), gamma polypeptide | 0.80 |
| NM_006169 | Nicotinamide N-methyltransferase (2×)3 | 2.57 | |
| Metabolism, energy | BC006229 | Cytochrome c oxidase subunit Vb | 0.78 |
| NM_001864 | Cytochrome c oxidase subunit VIIa polypeptide 1 | 0.78 | |
| NM_004541 | NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 1, 7.5 kDa | 0.78 | |
| BC005230 | Ubiquinol-cytochrome c reductase binding protein | 0.76 | |
| Metabolism, fatty acid and lipid | AB018580 | Aldo-keto reductase family 1, member C3 | 1.63 |
| RNA metabolism | H96549 | TIAI cytotoxic granule-associated RNA binding protein | 0.73 |
| NM_002933 | Ribonuclease, RNase A family, 1 | 1.43 | |
| Transcription | NM_003069 | SWI/SNF related, matrix associated | 0.69 |
| X72631 | Nuclear receptor subfamily 1, group D, member 1 | 0.77 | |
| Protein catabolism | NM_006762 | Lysosomal associated multispanning membrane protein 5 | 1.76 |
| NM_001909 | Cathepsin D | 1.47 | |
| AL573058 | Complement component 1, r subcomponent | 1.54 | |
| Protein synthesis | NM_024537 | Hypothetical protein FLJ12118 | 1.24 |
| Proliferation, positive | BC001188 | Transferrin receptor (p90, CD71) (2×)3 | 0.78 |
| NM_013407 | Deoxyhypusine synthase | 0.76 | |
| NM_020184 | Cyclin M4 | 0.79 | |
| Proliferation, negative | NM_001425 | Epithelial membrane protein 3 | 1.67 |
| AW089415 | Secreted frizzled-related protein 4 | 1.67 | |
| NM_022356 | Leucine proline-enriched proteoglycan (leprecan) 1 | 1.53 | |
| Binding proteins | AL117523 | Sterile alpha motif domain containing 4 | 0.80 |
| NM_004742 | BAI1-associated protein 1 | 1.78 | |
| NM_016303 | WW domain binding protein 5 | 1.53 | |
| Oxygen transport | AF349114 | Hemoglobin, beta (3×)3 | 0.65 |
| AF349571 | Hemoglobin, alpha 2 (6×)3 | 0.58 | |
| Unknown function | NM_017885 | Host cell factor C1 regulator 1 | 0.68 |
| BE739519 | Likely ortholog of mouse hypoxia induced gene 1 | 0.78 | |
| AL565741 | Hypothetical protein LOC90355 | 1.77 |
1
The unique identification number for the sequence.
2
The ratio of muscle transcript level at week 2 (during inadequate protein intake) to that at week 1 (during adequate protein intake) in the inadequate protein group (n = 11). A ratio > 1 represents up-regulation (ie, higher transcript level during week 2 than during week 1); a ratio < 1 represents down-regulation.
3
Indicates multiple probe sets for the same gene transcript; the number in parentheses after the name of the gene indicates the number of probe sets that had differential expression of the transcript.