TABLE 4.
Real-time polymerase chain reaction (RT-PCR) cross-validation of selected differentially expressed transcripts identified previously by microarray analysis1
| IP group (n = 11) |
|||
|---|---|---|---|
| Gene | Fold-change by microarray | Fold-change by RT-PCR | Control group: fold-change by RT-PCR (n = 10) |
| Lysosomal-associated multispanning membrane protein 5 | ↑ 1.5 | ↑ 2.0 | ↑ 2.6 |
| Ankyrin repeat domain | ↓ 1.1 | ↓ 1.6 | ↑ 1.2 |
| Cytochrome c oxidase subunit Vb | ↓ 1.3 | ↓ 1.2 | ↔1.0 |
| Hemoglobin, alpha 2 | ↓ 1.8 | ↓ 2.3 | ↑ 1.2 |
| Ras-related associated with diabetes | ↑ 3.0 | ↑ 3.7 | ↑ 1.6 |
| Metallothionein 1X | ↓ 1.6 | ↓ 1.5 | ↑ 1.2 |
| Collagen, type I, alpha 1 | ↑ 5.6 | ↑ 15.2 | ↑ 2.0 |
| Frizzled-related protein 4, secreted | ↑ 1.7 | ↑ 2.7 | ↑ 2.5 |
| CD163 antigen | ↑ 2.7 | ↑ 5.9 | ↑ 3.4 |
1
The inadequate protein (IP) group consumed adequate protein (1.2 g protein · kg-1 · d-1) during week 1 and inadequate protein (0.5 g protein · kg-1 · d1) during week 2; the control group consumed adequate protein (1.2 g protein · kg-1 · d-1) during weeks 1 and 2. Fold change 2/(transcript level at week 2/transcript level at week 1).