Figure 5. Human skin SCCs with Smad2 loss correlated with E-cadherin loss and nuclear Snail.

Skin SCCs were stained for Smad2 IHC (brown, top row), and immunofluorescence staining was performed for E-cadherin (green; middle row) and Snail (green; bottom row). A K14 antibody was used for immunofluorescent counterstain (red). An example of a pair SCCs from serial sections showed that a Smad2-positive SCC retained membrane-associated E-cadherin with a few Snail nuclear staining cells. In contrast, SCC with Smad2 loss lost membrane-associated E-cadherin but uniformly expressed Snail in the nucleus. Scale bar: 100 μm.