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. 2008 Jul 10;118(8):2733–2746. doi: 10.1172/JCI32381

Figure 2. Myosin isoform expression pattern in injured arteries after cuff placement in Lr11–/– mice.

Figure 2

(A) Sections of femoral arteries in Lr11+/+ or Lr11–/– mice after cuff placement, subjected to immunohistochemistry using antibodies against SMA or NMHCII-B. Arrowheads indicate the internal elastic layers. Scale bars: 50 μm. (B) mRNA levels of myosin isoforms NMHCII-B and SM1 in arteries after cuff placement in Lr11+/+ or Lr11–/– mice. Total RNA isolated from the thickened intima (I) or the media (M) of Lr11+/+ or Lr11–/– mice was reverse transcribed to cDNA and subjected to real-time PCR analysis using specific primers for NMHCII-B and SM1, respectively. The amounts of amplified products are expressed relative to the amounts of β-actin transcript. Data are presented as mean ± SD (n = 5). *P < 0.05.