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. 2008 Jul 10;118(8):2733–2746. doi: 10.1172/JCI32381

Figure 6. sLR11-mediated ruffle formation through complex formation with uPAR and integrin αvβ3.

Figure 6

(A) sLR11-induced membrane ruffle formation. Rabbit SMCs were incubated with sLR11 (1 μg/ml) for 24 hours in the presence or absence of antibody against LR11, uPAR, or integrin αvβ3 (MAB1976) or of PD98059. The number of cells with membrane ruffles were counted among 500 cells in the field. Data are presented as mean ± SD (n = 5). (B) Coimmunoprecipitation of sLR11 (~250 kDa) with integrin αvβ3 or uPAR. Membrane extracts of rabbit SMCs were incubated with or without sLR11 in the presence or absence of apoE (50 μg/ml) or RAP (10 μg/ml), immunoprecipitated with anti-integrin αvβ3 or anti-uPAR antibody, and subjected to immunoblot analysis using anti-LR11 antibody. (C) uPAR expression in Lr11–/– SMCs. Membrane extracts of Lr11+/+ or Lr11–/– SMCs were incubated with RAP and subjected to immunoblot analysis using anti-uPAR (~50 kDa) or anti-LRP1 (~85 kDa) antibody. Blot shown is representative of 3 independent experiments. Data are presented as mean ± SD (n = 3). *P < 0.05.