Skip to main content
. 2008 Jul 10;118(8):2733–2746. doi: 10.1172/JCI32381

Figure 7. sLR11-mediated intracellular signals related to cytoskeleton reorganization.

Figure 7

(A) sLR11-induced FAK activation. Cell lysates of rabbit SMCs were incubated with or without sLR11 (1 μg/ml) in the presence or absence of antibody against LR11 or integrin αvβ3 (MAB1976), immunoprecipitated with anti-FAK antibody, and subjected to immunoblot analysis using anti-FAK (~130 kDa) or anti–phospho-FAK (~130 kDa) antibody. (B) sLR11–induced phosphorylation of ERK1/2. Cell lysates (10 μg protein) of rabbit SMCs were incubated with sLR11 (1 μg/ml) for the indicated times in the presence or absence of anti–integrin αvβ3 antibody (MAB1976) and subjected to immunoblot analysis using antibody against (phospho) p42/44 MAP kinase. Upper and lower signals represent ERK1 (~44 kDa) and ERK2 (~42 kDa), respectively (13). Blot shown is representative of 3 independent experiments. Data of p-ERK1/2 are presented as mean ± SD (n = 3). *P < 0.05. (C) Rac1 activation in Lr11–/– SMCs. Cell lysates (60 μg protein) of Lr11+/+ or Lr11–/– SMCs were incubated with sLR11 (1 μg/ml) in the presence or absence of antibody against integrin αvβ3 (RMV-7), immunoprecipitated with PAK-1 PBD Protein GST beads, and subjected to immunoblot analysis with anti-Rac1 (~21 kDa) or anti–GTP-Rac1 (~21 kDa) antibody. (D) sLR11-induced Rac1 activation. Cell lysates (60 μg protein) of rabbit SMCs were incubated with sLR11 (1 μg/ml) for the indicated times in the presence or absence of anti–integrin αvβ3 antibody (MAB1976) and subjected to immunoblot analysis with anti-Rac1 (Rac1 ~21 kDa and GST-Rac1 ~21 kDa) antibody with (top) or without (bottom) prior immunoprecipitation with PAK-1 PBD Protein GST beads.