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. 2008 Jul 10;118(8):2733–2746. doi: 10.1172/JCI32381

Figure 8. Ang II–induced sLR11 production in SMCs.

Figure 8

(A) Effects of chemotactic cytokines on the production of sLR11 in rabbit SMCs. Conditioned media collected for 12 hours in the absence (lane 1) or presence of Ang II (1 μM, lane 2), PDGF (10 ng/ml, lane 3), or VEGF (50 ng/ml, lane 4) were concentrated and subjected to immunoblot analysis using anti-LR11 antibody (~250 kDa). Blot shown is representative of 3 independent experiments. Data are presented as mean ± SD (n = 3). *P < 0.05. (B) Ang II–dependent increase of soluble or membrane-bound forms of LR11 in SMCs. Membrane extracts (20 μg protein) prepared from rabbit SMCs or conditioned medium collected for 12 hours after the addition of Ang II at the indicated concentrations were subjected to immunoblot analysis with anti-LR11 (~250 kDa) or anti-LRP1 (~85 kDa) antibody. Blot shown is representative of 3 independent experiments. Data are presented as mean ± SD (n = 3). (C) Effect of ARBs or an ERK inhibitor on the Ang II–dependent increase in sLR11 in rabbit SMCs. Conditioned medium collected for 12 hours in the presence or absence of Ang II with or without valsartan (valsar, 10 nM), candesartan (10 nM), or PD98059 (10 μM) were subjected to immunoblot analysis with anti-LR11 (~250 kDa) antibody. Blot shown is representative of 3 independent experiments. Data are presented as mean ± SD (n = 3).