Figure 4.

Binding to the CRE by nuclear extract of odorant- or forskolin (FSK)-treated primary ORN cultures. Nuclear extracts from control, odorant, or forskolin-treated cells were prepared as described in Materials and Methods. The same amounts (10 μg) of total lysates were incubated with radiolabeled CRE probe for 15 min. For supershift assay, CRE–CREB complexes were further incubated with specific to anti-CREB or anti-pCREB Abs (α) or with preimmune serum (not shown). The CRE–CREB complex and supershifted complex are indicated by arrows. Results are representative of three independent experiments.