Fig. 5.

Hypoxic induction of CYC7 and OLE1 in strains carrying different subunit V isozymes. DW27 and DW29 cells were grown for six generations in midlogarithmic phase to steady state under normoxic conditions in a fermentor. The sparge gas was then changed from air to 97.5% N₂, 2.5% CO₂. Cells were harvested at the times indicated, their RNA was isolated, and gene expression was analyzed by Q-PCR using primers for CYC7, OLE1, and ACT1. Transcript levels from CYC7 and OLE1 were normalized to the transcript from ACT1, whose expression is not affected by O₂, and are expressed relative to their values at the beginning of the shift from normoxia to anoxia. Mean and standard deviation values are for three independent measurements.