Fig. 2.

In vitro DSR to H₂O₂ treatment. Primary rat glial cells, rat glioma cell lines (C6, A10-85, RG2, and 9L), a human glioma cell line (LN229), and a human neuroblastoma cell line (SH-SY5Y) were tested for glucose restriction-induced DSR. Cells were incubated in low glucose (0.5 g/liter, STS), normal glucose (1.0 g/liter), or high glucose (3.0g/liter), supplemented with 1% serum, for 24 h. Viability (MTT assay) was determined after a 24-h treatment with 0–1,000 μM H₂O₂. All data are presented as means ± SD. P values were calculated with Student's t test (*, P < 0.05; **, P < 0.01; ***, P < 0.001; of 0.5 and 1.0 g/liter vs. 3.0 g/liter glucose).