Fig. 4.
Glycosylation effect on the free energy of unfolding. (A) Free-energy profile against the fraction of native contacts (Q used as a reaction coordinate) for native SH3 (filled line) and for SH3 glycosylated with three (dotted line) or six (dashed line) 11-sugar ring glycans. (B) Kinetic effect of glycosylation as measured by the change in the height of the free-energy barrier of unfolding (at the Tf of the native SH3 domain). (C) Free-energy barrier of unfolding at the specific Tf of each glyco-conjugated variant of the SH3 domain (divided by the specific Tf).