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. 2008 Jun 11;105(24):8339–8344. doi: 10.1073/pnas.0801375105

Fig. 2.

Fig. 2.

NP-αGalCer immunization stimulates IgG anti-NIP. (A) Intraperitoneal administration of NP-αGalCer (0.5 μg per mouse) but not NP-KLH (2.2 μg per mouse), NP-βGalCer (0.5 μg per mouse), or αGalCer (0.5 μg per mouse) stimulates early anti-NIP IgG in vivo. (B) IgG anti-NIP from mixed splenic B1–8hi B and iNK T TcR Tg T cell culture supernatants 7 days after media, 15 μg/ml αIgM, 1 μg/ml LPS, 0.2 μg/ml αCD3, 8.6 μg/ml NP-KLH, 1 ng/ml αGalCer, 1 ng/ml NP-αGalCer, or 1 ng/ml NP-βGalCer stimulation (*, significantly different from PBS/media group).