Fig. 1.

Free diffusion measurements. (A) Representative images after ejection of Lf or Tf, with or without H, into dilute agarose. (Scale bars: 200 μm.) (B) Fluorescence intensity (I) profiles were extracted from each image (data in blue) and fit to the diffusion equation (fits in red) along a single axis (depicted in A at the far left). (C) Linear regression of data in A and B; γ_i² = 4D(t_i + t₀), so regression of γ_i²/4 on t_i returns a slope of D (data transformed to a zero y-intercept). Fitting yielded the following D values: Lf, 6.5 × 10⁻⁷ cm²·s⁻¹; Lf + H, 4.6 × 10⁻⁷ cm²·s⁻¹; Tf, 7.2 × 10⁻⁷ cm²·s⁻¹; and Tf + H, 7.9 × 10⁻⁷ cm²·s⁻¹. (D) Summary data (mean ± SD; *, P < 0.0002, ANOVA).