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. 2008 Jun 16;105(24):8440–8445. doi: 10.1073/pnas.0803096105

Fig. 3.

Fig. 3.

Antisecretory activity of BPIPP in cell culture and in vivo. (A) T84 cells were pretreated with 50 μM BPIPP or vehicle in solution 1 containing NaCl, and chloride transport was measured in the presence of indicated concentrations of STa in solution 2 containing NaNO3 by using 6-methoxy-1-(3-sulfonatopropyl)quinolinium (SPQ) fluorescence as a Cl− sensor. n = 8. (B) Effect of 50 μM BPIPP on Cl− transport in T84 cells stimulated by various agents in solution 1 containing NaCl; 100 μM isoproterenol (Iso), 10 μg/ml cholera toxin (CT; CT-ptr, BPIPP was present during pretreatment similar to Fig. 2B; CT-inc, BPIPP was present only during incubation and assay phase; CT-both, BPIPP was present during pretreatment and incubation-assay phase), 25 μM forskolin (For), 1 mM or 0.1 mM 8-bromo-cAMP (BcA-1 or BcA-0.1), or 10 μM ionomycin (Iono). Fluorescence was measured by using SPQ or N-(ethoxycarbonylmethyl)-6-methoxyquinolinium bromide (MQAE) as indicator. Absolute Ft − Fc fluorescence values for vehicle control were 138 ± 6 for isoproterenol, 74 ± 5 for cholera toxin, 1,031 ± 36 for forskolin, 52 ± 2 and 780 ± 84 for 0.1 and 1 mM 8-bromo-cAMP in the presence of SPQ, and 635 ± 75 and 1,945 ± 102 for 0.1 and 1 mM 8-bromo-cAMP and 720 ± 81 for ionomycin in the presence of MQAE. *, P < 0.05 BPIPP vs. vehicle; n = 8. (C) Effect of compounds on secretion in rabbit intestinal segments (ileal loops) injected with 1 ml of PBS containing indicated concentrations of STa [none (Control), 0.1, 0.2, or 1.0 μM] with vehicle, 10 or 50 μM BPIPP, and 50 μM compound IVa. Incubations were continued for 5 h, and then volume/length ratios of the segments were determined. *, P < 0.05; n = 4–8.