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. 2008 Jun 16;105(24):8440–8445. doi: 10.1073/pnas.0803096105

Fig. 4.

Fig. 4.

Stimulation of activity of a tyrosine-specific protein kinase in T84 cells by BPIPP. (A) Cells were treated with 50 μM BPIPP or vehicle for 20 min and protein kinase activity was measured in the extract by using various tyrosine-containing protein kinase substrates. E4Y, (Glu)4-Tyr; EAY, Glu-Ala-Tyr; EY, Glu-Tyr. n = 3. (B) Cells were pretreated in a similar manner and then homogenized with a glass–glass homogenizer in a hypotonic buffer and fractionated to separate crude membrane fraction from the cytosol. Protein kinase activity was measured by using EAY as the substrate. *, P < 0.05; n = 6.