Figure 1.

Thioredoxin h constructs used for barley transformation. (Upper) pdBhWTRXhN-1 contains wtrxh under control of the barley endosperm-specific B₁-hordein promoter without its signal peptide sequence and terminated by nos. (Lower) pdBhssWTRXhN3–8 contains wtrxh under control of the B₁-hordein promoter with its signal peptide sequence and terminated by nos. Nucleotide sequences of the PCR-amplified wheat thioredoxin h coding region and B₁-hordein 5′ flanking region were determined by DNA sequencing.