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. 1999 Dec 7;96(25):14641–14646. doi: 10.1073/pnas.96.25.14641

Figure 4.

Figure 4

Western blot analysis of extracts of T2 and T3 barley grain transformed with wtrxh. Forty micrograms of soluble proteins extracted from 10–20 grains of each line was fractionated by SDS/PAGE. Lane 1, wheat germ thioredoxin h; lane 2, nontransgenic control of GP 4–96; lane 3, null segregant T3 grain of GPdBhssBarWtrx-29–11–10; lane 4, heterozygous T1 grain of GPdBhssBarWtrx-29; lane 5, homozygous T2 grain of GPdBhssBarWtrx-29–3; lane 6, homozygous T3 grain of GPdBhssBarWtrx-29–3-2; lane 7, prestained standards: aprotinin, 6.9 kDa; lysozyme, 17.8 kDA; soybean trypsin inhibitor, 30.6 kDA; carbonic anhydrase, 41.8 kDa; BSA, 7l kDa.