Table 2.

Western blot analysis of overexpression of wheat thioredoxin h in transgenic barley grain

Transgenic barley line	Fold overexpression
Nontransformed control
GP 4-96	1.0*
Transformant with wtrxh†
GPdBhBarWtrx-2	6.4
Transformants with wtrxh and signal sequence‡
GPdBhssBarWtrx-7	5.5
GPdBhssBarWtrx-22	22.0
GPdBhssBarWtrx-29	10.0
Null segregant§
GPdBhssBarWtrx-29-11-10	0.64

^*Corresponds to 1.46% of total volume scanned. 

^† pdBhWTRXhN-1. 

^‡ pdBhssWTRXhN3-8. 

^§ Originated from GPdBhssBarWtrx-29.