Table 1.

Yeast strains

Strain	Construct*	Parent vector	ACC1	URA3	LEU2	HIS3	TRP1	Galactose dependence	Doubling time, hr†		Herbicide sensitivity	Ref.
									23° C	30° C
MJ 1.12	C100	pRS424	−	−	+	−	+	+	4.8  ± 0.2	3.1  ± 0.1	No	18
MJ 1.13	C100	pRS424	−	−	+	−	+	+	4.6  ± 0.2	3.2  ± 0.1	No	18
MJ 9.11	Yeast ACC1§	pRS426	+	+	+	−	−	−	4.5  ± 0.2	2.2  ± 0.2	No	18
MJ 9.14	Yeast ACC1§	pRS426	+	+	+	−	−	−	5.3  ± 0.4	2.4  ± 0.1	No	18
MJ 9.13	Yeast ACC1¶	None	+	−	−	−	−	−	3.9  ± 0.2	2.4  ± 0.1	No	18
TN 1/2-1	C50P50	pRS423	−	−	+	+	−	+	6.0  ± 0.3	4.3  ± 0.2	Yes	This work
TN 1/2-3	C50P50	pRS423	−	−	+	+	−	+	4.7  ± 0.2	4.4  ± 0.1	Yes	This work
PG 3.34	C80P20	pRS423	−	−	+	+	−	+	8.9  ± 0.3	13.3  ± 1.7	No	This work
PG 3.43	C80P20	pRS423	−	−	+	+	−	+	8.8  ± 0.2	13.5  ± 1.8	No	This work
PG 4.41	C60P40	pRS423	−	−	+	+	−	+	14.6  ± 1.2	ND	Yes	This work
PG 4.43	C60P40	pRS423	−	−	+	+	−	+	12.4  ± 0.9	ND	Yes	This work
PG 5.21	C50P10C20P20	pRS423	−	−	+	+	−	+	9.2  ± 0.3	ND	No	This work
PG 5.22	C50P10C20P20	pRS423	−	−	+	+	−	+	14.3  ± 1.0	ND	No	This work

All strains were obtained by sporulation of W303D-ACC1^ΔLeu2 transformed with an appropriate plasmid. Haploid strains: 1.12 and 1.13, 9.11 and 9.14, 4.41 and 4.43, 5.21 and 5.22 were obtained from single tetrads. W303D-ACC1^ΔLeu2 relevant genotype: MATa/MATα, leu2-3, 112/leu2-3, 112 his3-11,15/his311, 15 ade2-1/ade2-1 ura3-1/ura3-1 trp-1/trp1-1 can1-100/can1-100 ACC1/acc1∷LEU2. 

^*Structures of the constructs are shown in Fig. 1, except ACC1, which are annoted below. 

^† In YPRG medium; ND, no growth detected. 

^‡ Strong sensitivity to aryloxyphenoxypropionate and cyclohexanedione herbicides. 

^§ Plasmid-borne. 

^¶ Genomic.