Fig. 4.

A study to assess the effects of β1-integrin knockdown by RNAi on the steady-state levels of junction-associated proteins in primary Sertoli cell and Sertoli–germ cell cocultures. (A–B) Immunoblots showing changes in the steady-state levels of several junction proteins after β1-integrin RNAi in Sertoli cell cultures (A) or Sertoli–germ cell cocultures (B), with actin serving as a loading control. From both sets of cultures, lysates were used for immunoblot analysis as shown in A and B, and data from three experiments were scanned and shown in C–H. Each bar is the mean ± SD (n = 3) and normalized against actin, wherein control cultures were arbitrarily set at 100%, against which one-way ANOVA was performed. ns, not significantly different; ∗, P < 0.01.