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. 2008 Jun 24;105(26):9000–9005. doi: 10.1073/pnas.0800057105

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© 2008 by The National Academy of Sciences of the USA

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Fig. 4. — Loss of H3 K56ac promotes transcriptional repression. (A) Histone H3 levels over the PHO5 promoter relative to the GAL1 control region; addition of phosphate promotes repression. (B) H3 K56ac levels over the PHO5 promoter relative to the GAL1 control region. (C) Histone H3 K56ac levels, normalized to H3 levels, over the PHO5 promoter relative to the GAL1 control region. Samples were taken from the same time course in A–C. (D) Acid phosphatase levels were measured in strains that are WT, K56Q, and K56R versions of MSY421. (E) Model for the role of H3 K56 acetylation in promoter chromatin disassembly during transcriptional induction, and loss of K56 acetylation in chromatin reassembly during transcriptional repression.