Fig. 2.
(E)-BCP and (Z)-BCP displace [3H]CP-55,940 from hCB2 receptors expressed in HEK293 cells. (A) Sigmoidal displacement curves (R2 = 0.93 for (E)-BCP and 0.98 for (Z)-BCP) show overall displacement (71% and 84%, respectively) with Ki values of 155 and 485 nM, respectively. Data show mean values of nine measurements ± SEM. (B) Hill plot showing linearized data and the corresponding Ki values. (C) Dixon plot of the competitive binding interaction of (E)-BCP with the CP-55,940 receptor binding site in the CB2 receptor. Radioligand assays were performed by using 84 pM, 168 pM, and 252 pM [3H]CP-55,940.