Figure 3.

Secondary-structure model of synthetic MFA2 T7A18 mRNA. The moderate-to-strong cleavage sites found with the three RNases and the same sites of chemical modification by DMS and CMC were used as constraints with the mfold program package. These sites are superimposed on the structural model. Short stems found with mfold, but not detected by the probing results, are not included in the model. The numbers indicate the nucleotide positions relative to the 5′ end. The figure was drawn with the help of loopdloop [Gilberg, D. G. (1992), available via anonymous ftp from ftp.bio.indiana.edu].