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Binding isotherm for mjaSSB with (dT)30. Binding reaction mixtures (20 μl) contained 0.03 fmol of [5′-32P]dT30 and the indicated concentration of mjaSSB. Protein–DNA complexes were separated from free DNA by agarose gel electrophoresis, and the radioactivity in each species was determined by using a phosphorimager. The apparent Ka is 2 × 1010 M−1.
